The ERBA Molecular development team has extensive experience of a number of amplification technologies but with a particular speciality in isothermal systems.
When these isothermal systems are used in conjunction with the BART (real-time detection) technology it has enabled the development of the simplest ever readers for real-time closed tube assays.
Next generation isothermal amplification technologies
ERBA Molecular has developed a number of key improvements to isothermal amplification technologies increasing their robustness, functionality, speed and applications. The proprietary “STEM” technology, for example, has led not only to significant improvements in existing technologies but is enabling entirely novel isothermal methods.
ERBA Molecular has developed at technology called STEMS that can be incorporated into some isothermal amplification systems significantly improving performance and versatility. Isothermal amplification technologies can offer highly specific and sensitive alternatives to PCR that do not require thermo-cycling.
The addition of STEM technology to LAMP allows very flexible primer design and simplifies development of complex molecular assays which in turn lowers development cycle times and leads to lower R&D cost.
The LAMP technology was much improved by the addition of Loop Primers which accelerate the rate of amplification. However, the positioning and orientation of Loop Primers relative to the other priming sites used in LAMP is highly restrictive.
The Stem technology also allows for multiple labels to be targeted on a single amplicon so facilitating multi-plex typing of pathogens.
The STEM-primers bind to the “Stem” of the Stem-Loop structures characteristic of LAMP that become transiently single-stranded during replication. One or more “Stem-Primers” can be used and, unlike Loop Primers, they can be used in either orientation.
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